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prk5 jak1  (Addgene inc)


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    Structured Review

    Addgene inc prk5 jak1
    CRTCs can bind STAT3, STAT5 and MDM2. ( a ) 293T cells were transfected with FLAG-tagged CRTCs and/or STATs. FLAG-immunoprecipitates (IPs) were analyzed for STAT3 and STAT5 by western blotting. ( b ) 293T cells were transfected with MYC-tagged MDM2 and/or STATs. MYC-IPs were analyzed for STAT3 and STAT5 by western blotting. ( c ) 293T cells were transfected with MYC-tagged MDM2 and/or <t>JAK1.</t> MYC-IPs were analyzed for JAK1 by western blotting. ( d ) 293T cells were transfected with FLAG-tagged CRTC2 and/or MDM2. FLAG-IPs were analyzed for MDM2 by western blotting. ( e ) 293T cells were transfected with FLAG-tagged CRTC3 and/or MDM2. FLAG-IPs were analyzed for MDM2 by western blotting
    Prk5 Jak1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prk5+jak1/pcDNA+FLAG+TORC3+(Plasmid+%2322976)/pmc05108339-125-47-62
    Average 92 stars, based on 3 article reviews
    prk5 jak1 - by Bioz Stars, 2026-11
    92/100 stars

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    1) Product Images from "MDM2 facilitates adipocyte differentiation through CRTC-mediated activation of STAT3"

    Article Title: MDM2 facilitates adipocyte differentiation through CRTC-mediated activation of STAT3

    Journal: Cell Death & Disease

    doi: 10.1038/cddis.2016.188

    CRTCs can bind STAT3, STAT5 and MDM2. ( a ) 293T cells were transfected with FLAG-tagged CRTCs and/or STATs. FLAG-immunoprecipitates (IPs) were analyzed for STAT3 and STAT5 by western blotting. ( b ) 293T cells were transfected with MYC-tagged MDM2 and/or STATs. MYC-IPs were analyzed for STAT3 and STAT5 by western blotting. ( c ) 293T cells were transfected with MYC-tagged MDM2 and/or JAK1. MYC-IPs were analyzed for JAK1 by western blotting. ( d ) 293T cells were transfected with FLAG-tagged CRTC2 and/or MDM2. FLAG-IPs were analyzed for MDM2 by western blotting. ( e ) 293T cells were transfected with FLAG-tagged CRTC3 and/or MDM2. FLAG-IPs were analyzed for MDM2 by western blotting
    Figure Legend Snippet: CRTCs can bind STAT3, STAT5 and MDM2. ( a ) 293T cells were transfected with FLAG-tagged CRTCs and/or STATs. FLAG-immunoprecipitates (IPs) were analyzed for STAT3 and STAT5 by western blotting. ( b ) 293T cells were transfected with MYC-tagged MDM2 and/or STATs. MYC-IPs were analyzed for STAT3 and STAT5 by western blotting. ( c ) 293T cells were transfected with MYC-tagged MDM2 and/or JAK1. MYC-IPs were analyzed for JAK1 by western blotting. ( d ) 293T cells were transfected with FLAG-tagged CRTC2 and/or MDM2. FLAG-IPs were analyzed for MDM2 by western blotting. ( e ) 293T cells were transfected with FLAG-tagged CRTC3 and/or MDM2. FLAG-IPs were analyzed for MDM2 by western blotting

    Techniques Used: Transfection, Western Blot

    Related Articles

    Plasmid Preparation:

    Article Title: MDM2 facilitates adipocyte differentiation through CRTC-mediated activation of STAT3
    Article Snippet: .. pBABE-puro was a generous gift from Dr Ormond MacDougald. pBABE-puro C/EBPδ was described previously. pcDNA3 was kindly provided by Dr Jean-Christophe Marine. pCMV FLAG-MYC-CRTC2 was donated by Dr. Jeffrey Meier. pcDNA FLAG CRTC3 was a gift from Dr. Marc Montminy (Addgene, Cambridge, MA, USA; plasmid # 22976). pRK5 JAK1 was used previously. pcDNA3 STAT3 was a generous gift from Dr Jim Darnell (Addgene; plasmid #8706). ..



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    prk5 jak1  (Addgene inc)
    92
    Addgene inc prk5 jak1
    CRTCs can bind STAT3, STAT5 and MDM2. ( a ) 293T cells were transfected with FLAG-tagged CRTCs and/or STATs. FLAG-immunoprecipitates (IPs) were analyzed for STAT3 and STAT5 by western blotting. ( b ) 293T cells were transfected with MYC-tagged MDM2 and/or STATs. MYC-IPs were analyzed for STAT3 and STAT5 by western blotting. ( c ) 293T cells were transfected with MYC-tagged MDM2 and/or <t>JAK1.</t> MYC-IPs were analyzed for JAK1 by western blotting. ( d ) 293T cells were transfected with FLAG-tagged CRTC2 and/or MDM2. FLAG-IPs were analyzed for MDM2 by western blotting. ( e ) 293T cells were transfected with FLAG-tagged CRTC3 and/or MDM2. FLAG-IPs were analyzed for MDM2 by western blotting
    Prk5 Jak1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prk5+jak1/pcDNA+FLAG+TORC3+(Plasmid+%2322976)/pmc05108339-125-47-62
    Average 92 stars, based on 1 article reviews
    prk5 jak1 - by Bioz Stars, 2026-11
    92/100 stars
    		  Buy from Supplier

    Image Search Results


    CRTCs can bind STAT3, STAT5 and MDM2. ( a ) 293T cells were transfected with FLAG-tagged CRTCs and/or STATs. FLAG-immunoprecipitates (IPs) were analyzed for STAT3 and STAT5 by western blotting. ( b ) 293T cells were transfected with MYC-tagged MDM2 and/or STATs. MYC-IPs were analyzed for STAT3 and STAT5 by western blotting. ( c ) 293T cells were transfected with MYC-tagged MDM2 and/or JAK1. MYC-IPs were analyzed for JAK1 by western blotting. ( d ) 293T cells were transfected with FLAG-tagged CRTC2 and/or MDM2. FLAG-IPs were analyzed for MDM2 by western blotting. ( e ) 293T cells were transfected with FLAG-tagged CRTC3 and/or MDM2. FLAG-IPs were analyzed for MDM2 by western blotting

    Journal: Cell Death & Disease

    Article Title: MDM2 facilitates adipocyte differentiation through CRTC-mediated activation of STAT3

    doi: 10.1038/cddis.2016.188

    Figure Lengend Snippet: CRTCs can bind STAT3, STAT5 and MDM2. ( a ) 293T cells were transfected with FLAG-tagged CRTCs and/or STATs. FLAG-immunoprecipitates (IPs) were analyzed for STAT3 and STAT5 by western blotting. ( b ) 293T cells were transfected with MYC-tagged MDM2 and/or STATs. MYC-IPs were analyzed for STAT3 and STAT5 by western blotting. ( c ) 293T cells were transfected with MYC-tagged MDM2 and/or JAK1. MYC-IPs were analyzed for JAK1 by western blotting. ( d ) 293T cells were transfected with FLAG-tagged CRTC2 and/or MDM2. FLAG-IPs were analyzed for MDM2 by western blotting. ( e ) 293T cells were transfected with FLAG-tagged CRTC3 and/or MDM2. FLAG-IPs were analyzed for MDM2 by western blotting

    Article Snippet: pBABE-puro was a generous gift from Dr Ormond MacDougald. pBABE-puro C/EBPδ was described previously. pcDNA3 was kindly provided by Dr Jean-Christophe Marine. pCMV FLAG-MYC-CRTC2 was donated by Dr. Jeffrey Meier. pcDNA FLAG CRTC3 was a gift from Dr. Marc Montminy (Addgene, Cambridge, MA, USA; plasmid # 22976). pRK5 JAK1 was used previously. pcDNA3 STAT3 was a generous gift from Dr Jim Darnell (Addgene; plasmid #8706).

    Techniques: Transfection, Western Blot